RSNA 2010 

Abstract Archives of the RSNA, 2010


SSA21-03

Sonoactived Doxorubicin Loaded Nanoliposomes

Scientific Formal (Paper) Presentations

Presented on November 28, 2010
Presented as part of SSA21: Physics (Ultrasound)

Participants

Saujanya Laxmirajam Gosangari PhD, Abstract Co-Author: Researcher, Banner Pharmaceutical
Kenneth Lloyd Watkin MS, PhD, Presenter: Stockholder, Ultra-Imaging LLC Stockholder, SonoActive Inc

CONCLUSION

This research demonstrates that medical diagnostic ultrasound energy levels have the capability to enhance release from specially formulated nanoliposomes, wherein the release is a function of the lipid composition, irradiation times and power intensity level.

BACKGROUND

 Liposomes as drug delivery carriers have been pursued for several decades. Anticancer drugs have been formulated in lipid vesicles to achieve high encapsulation coupled with resistance to leakage and drug loss while in circulation. Surface chemistry can be employed to modify the liposome surface to carry specialized molecules and proteins, which further enhances the functionality of these liposomes.  Liposomal formulations of anthracyclines have been used extensively for cancer treatment to facilitate increased drug accumulation and uptake at the tumor site. Once the drug is incorporated into the liposome and has reached the tumor site, the biggest challenge is the release of the contents. In this research we report on a modified medical diagnostic ultrasound system that has been utilized with specially formulated nanoliposomes to demonstrated the effectiveness of sonoactive delivery of  doxorubicin nanoliposomes.

EVALUATION

A specially modified 7.5 MHz diagnostic ultrasound system (Aloka systems) was used for the release experiments.  The nanoliposomes were irradiated for four different pulse repetition times; 100, 250, 500, and 1000 microseconds. The power intensity levels and the time of irradiation were also varied The ultrasound sensitive nanoliposomes released increasing amounts of drug in response to increasing irradiation times and energy levels while the drug release was not significant when the non-ultrasound sensitive nanoliposomes were exposed to ultrasound energy levels. In vitro studies to test the cytotoxicity of the formulations demonstrateded that doxorubicin loaded nanoliposomes significantly inhibited cell survival of oral cancer cells when compared to the non-ultrasound sensitive nanoliposomes.

DISCUSSION

This research highlights the need to control not only the ultrasound parameters for sonoactivation but also the need for special formulations of lipid nanoparticles for ultrsound mediated delivery.

Cite This Abstract

Gosangari, S, Watkin, K, Sonoactived Doxorubicin Loaded Nanoliposomes.  Radiological Society of North America 2010 Scientific Assembly and Annual Meeting, November 28 - December 3, 2010 ,Chicago IL. http://archive.rsna.org/2010/9013031.html