Yoshikazu Okamoto MD, Presenter: Nothing to Disclose

Tomonori Isobe PhD, Abstract Co-Author: Nothing to Disclose

Yuji Hirano, Abstract Co-Author: Nothing to Disclose

Kiichi Tadano, Abstract Co-Author: Nothing to Disclose

Shintrao Mori, Abstract Co-Author: Nothing to Disclose

Manabu Minami MD, PhD, Abstract Co-Author: Nothing to Disclose

To elucidate the usefulness of 3T H-MR spectroscopy (H-MRS) by observing the change of intramyocellular lipid metabolism due to successive skeletal muscle training for 3 months

Six healthy males in their 20s to 30s were recruited for this study. All volunteers performed muscle training (“calf raise”) stressing the triceps surae muscles of bilateral calves. One-time training involved 3 sets of 10 times calf raising exercise bilaterally in turns. All volunteers performed this training twice a week for 3 continuous months. We acquired H-MRS data of these bilateral twelve soleus (SOL) muscles of the calf by 3T clinical MRI. First, we performed the baseline H-MRS study before starting training, and scanned every 2 weeks (7 times) after starting training. There was an interval of at least 48 hours from the training to MRI scan in all examinations. All MRS data of soleus muscles were analyzed by LC-model technique (muscle mode).  

In comparison between pre-training and after the 3 months training, the average of the maximal dimension area of the calf of 12 calves was not significantly changed. However, the maximal muscle strength was apparently increased in isometric (2.28 double) and isotonic (1.81 double) measurements. The average amount of IMCL of 12 calves at pre-training state was 645 water scaling value of the LCModel. Although the average amount of IMCL contents had not showed a significant change early in the first month (2nd scan), it had gradually decreased, and eventually, it showed 500 water scaling value of LCModel at the final scanning. Wilcoxon signed-rank test showed statistically significant difference between pre and post (0.02<P<0.05), and 2nd and post (P<0.01). EMCL showed no characteristic change during the training.   

From these results, we successfully observed the effects of muscle training in reducing the intramyocellular lipid content using 3T H-MRS.  

3T H-MRS has sufficient potential to evaluate the measurement of IMCL, which can apply to the assessment of fat metabolism including insulin resistance.

Okamoto, Y, Isobe, T, Hirano, Y, Tadano, K, Mori, S, Minami, M, Evaluation of the Change of Intramyocellular Lipid Metabolism due to Long-term Skeletal Muscle Training of the Calf by 3 T H-MR Spectroscopy.  Radiological Society of North America 2010 Scientific Assembly and Annual Meeting, November 28 - December 3, 2010 ,Chicago IL. http://archive.rsna.org/2010/9002342.html