Sheng Hong Ju MD, Presenter: Nothing to Disclose

Gaojun Teng MD, Abstract Co-Author: Nothing to Disclose

Xi Mao PhD, Abstract Co-Author: Nothing to Disclose

To label human umbilical cord blood mesenchymal stem cells(MSCS) by combining Poly-L-Lysine(PLL) with magnetic iron oxide and to obtain 1.5T MR images.

Poly-L-Lysine(PLL) was combined with magnetic iron oxide called Fe2O3-PLL. Human umbilical cord blood MSCS were isolated, purified, expanded and then incubated with Fe2O3-PLL. Prussian blue stain was performed for showing intracellular iron. For evaluating toxicity and proliferation of various concentration Fe2O3-PLL labeled MSCS, MTT assay was assessed. The cells apoptosis rate was determined by AnnexinV / PI double staining method. Cells underwent MR imaging with T1WI、T2WI、T2*WI.

Iron-containing intracytoplasmatic vesicles could be observed clearly with prussian blue staining. Among various concentrations Fe2O3-PLL labeled MSCS and unlabeled cells, MTT value of light absorption had no statistical significant difference (Kruskal-Wallis test, χ2=10.35, P=0.17). 20μg/ml of iron was the suitable concentration for incubating cells. The early apoptotic cells(AnnexinV-FITC positive / PI negative) of labeled and unlabeled MSCS were 9.93%, 10.14% respectively, late apoptotic cells(AnnexinV-FITC positive / PI positive) were 5.43%、2.95%. The signal intensity decrease for 1×106, 5×105 labeled cells compared with that for unlabeled cells was demonstrated on T1WI, T2WI, T2*WI. The percentage change in signal intensity(∆SI) was larger for 106 labeled cells than that for 5×105 labeled cells especially on T2*WI. After 8 days’ culture, ∆SI for 106 labeled MSCS decreased compared with that for the same cells 7 days ago.

The human umbilical cord blood MSCS can be labeled with Fe2O3-PLL without significant change in viability and apoptosis. The labeled MSCS can be imaged with standard 1.5 Tesla MR equipment.

To label human umbilical cord blood mesenchymal stem cells(MSCS) by combining Poly-L-Lysine(PLL) with magnetic iron oxide and to obtain 1.5T MR images.

Poly-L-Lysine(PLL) was combined with magnetic iron oxide called Fe2O3-PLL. Human umbilical cord blood MSCS were isolated, purified, expanded and then incubated with Fe2O3-PLL. Prussian blue stain was performed for showing intracellular iron. For evaluating toxicity and proliferation of various concentration Fe2O3-PLL labeled MSCS, MTT assay was assessed. The cells apoptosis rate was determined by AnnexinV / PI double staining method. Cells underwent MR imaging with T1WI、T2WI、T2*WI.

Iron-containing intracytoplasmatic vesicles could be observed clearly with prussian blue staining. Among various concentrations Fe2O3-PLL labeled MSCS and unlabeled cells, MTT value of light absorption had no statistical significant difference (Kruskal-Wallis test, χ2=10.35, P=0.17). 20μg/ml of iron was the suitable concentration for incubating cells. The early apoptotic cells(AnnexinV-FITC positive / PI negative) of labeled and unlabeled MSCS were 9.93%, 10.14% respectively, late apoptotic cells(AnnexinV-FITC positive / PI positive) were 5.43%、2.95%. The signal intensity decrease for 1×106, 5×105 labeled cells compared with that for unlabeled cells was demonstrated on T1WI, T2WI, T2*WI. The percentage change in signal intensity(∆SI) was larger for 106 labeled cells than that for 5×105 labeled cells especially on T2*WI. After 8 days’ culture, ∆SI for 106 labeled MSCS decreased compared with that for the same cells 7 days ago.

The human umbilical cord blood MSCS can be labeled with Fe2O3-PLL without significant change in viability and apoptosis. The labeled MSCS can be imaged with standard 1.5 Tesla MR equipment.

Ju, S, Teng, G, Mao, X, In Vitro Labeling and MR Imaging of Mesenchymal Stem Cells from Human Umbilical Cord Blood.  Radiological Society of North America 2005 Scientific Assembly and Annual Meeting, November 27 - December 2, 2005 ,Chicago IL. http://archive.rsna.org/2005/4406179.html