yaoping shi, Presenter: Nothing to Disclose

Xiaoming Yang MD, PhD, Abstract Co-Author: Nothing to Disclose

Feng Zhang MD, PhD, Abstract Co-Author: Nothing to Disclose

zhibin bai, Abstract Co-Author: Nothing to Disclose

Jianfeng Wang MD, Abstract Co-Author: Nothing to Disclose

Long-Hua Qiu, Abstract Co-Author: Nothing to Disclose

Yanfeng Meng MD, Abstract Co-Author: Nothing to Disclose

To determine whether radiofrequency (RF) hyperthermia could enhance the therapeutic effect of cisplatin and 5-fluorouracil (5-FU) on esophageal squamous cancers (ESC).

Human ESC cells (ESCC) were first labeled with red fluorescent protein (RFP) via a lentivirus transfection approach. For both in vitro confirmation and in vivo validation studies, RFP-ESCCs and 24 RFP-ESC-engrafted mice were divided into four study groups with various treatments of (i) combination therapy with chemotherapy (cisplatin and 5-FU) plus MR imaging-heating-guidewire (MRIHG)-mediated local RF hyperthermia; (ii) chemotherapy only; (iii) RF hyperthermia only; and (iv) phosphate-buffered saline (PBS). In vitro cell proliferation was quantified by MTS assay, while in vivo validation with size changes of ESC masses and RFP-ESC signals among different treatment groups were monitored by ultrasound imaging and optical imaging over time with subsequent pathology correlation.

Of in vitro experiments, MTS assay demonstrated lowest cell proliferation of combination therapy compared to those of three control groups (41±6% VS 59±4% VS 92± 2% VS 100±2%, p < 0.05). Of in vivo experiments, ultrasound imaging showed smaller tumor volumes with combination therapy than those with three control treatments (0.55±0.07mm3 VS 1.28±0.07mm3 VS 2.42±0.45mm3 VS 2.67±0.39mm3). Optical imaging demonstrated a decrease of RFP-ESC signals for the combination therapy group in comparison to those for three control groups (0.61±0.16 photon/sec/mm2 VS 1.31±0.13photon/sec/mm2 VS 2.08±0.43 photon/sec/mm2 VS 2.69±0.26photon/sec/mm2), which were correlated with histologic confirmation.

Local RF hyperthermia can enhance chemotherapeutic effect on human ESCs, which may open a new avenue for efficient management of esophageal malignancies.

Local RF hyperthermia can enhance chemotherapeutic effect on human ESCs, which may open a new avenue for efficient management of esophageal malignancies.

shi, y, Yang, X, Zhang, F, bai, z, Wang, J, Qiu, L, Meng, Y, Radiofrequency Hyperthermia-Enhanced Local Chemotherapy of Esophageal Squamous Cancers: Monitoring with Dual-Modality Imaging.  Radiological Society of North America 2014 Scientific Assembly and Annual Meeting, - ,Chicago IL. http://archive.rsna.org/2014/14014882.html