Shui Xing Zhang MD, Presenter: Nothing to Disclose

Wen-Bo Chen BArch, Abstract Co-Author: Nothing to Disclose

Chang Hong Liang MD, Abstract Co-Author: Nothing to Disclose

To investigate noninvasive blood oxygen level-dependent imaging (BOLD) sequences for measuring renal function in contrast induced acute kidney injury (CIAKI) rats after the administration of iodinated contrast media (CM) and further correlate with the expression of AQP1.

 Thirteen male Sprague-Dawley (SD) rats with weight of 200-250 g were randomized grouped to a CIAKI group (injected with Meglumine Diatrizoate, 370mg/ml, 6 ml/kg body weight) and a control group (injected with same amount of 0.9% saline). All procedures were approved by the local Research Ethics Committee, and in accordance with the Guide of the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996). BOLD sequences were performed at 24 h pre-injection and at intervals of 30 min, 12 h, 24 h, 48 h, 72 h and 96 h post-injection to assess renal relative spin–spin relaxation rate (R2*) respectively. At each time point, 3 rats were executed and the kidneys were performed immunohistochemistry(IHC) for measuring the expression of AQP1. Data were analyzed using SPSS 13.0 for Windows. One-way ANOVA test and Bivariate Correlations were used. P<0.05 was considered as statistical difference.

For the CIAKI group, the values of R2* in the outer medulla (OM) of kidneys were markedly increased at 30 min, 12 h, 24 h and 48 h (P<0.05 vs. baseline, n=6), respectively; whereas the changes at 72 h and 96 h in the OM and in the cortex and inner medulla (IM) were not statistically significant (P=NS vs. baseline). Besides, the expression of AQP1 at 30 min, 12 h, 24 h and 48 h (P<0.05 vs. baseline, n=3) were higher than control group. At 30 min to 48 h, the correlation coefficient r between R2* and AQP1 were 0.575 (P = 0.025, one-tailed).

The increase of oxygen level in OM were found at 30 min-48 h post-injection of iodinated CM. BOLD sequence provides means for noninvasive monitoring renal function during the first 2 days of CIAKI in clinical routine works. The AQP1 expression was positively correlated with R2 *. Therefore BOLD may be a objective mean for forecasting AQP1.

BOLD may provides means for noninvasive monitoring renal function of CIAKI in clinical routine works and become a objective mean for forecasting AQP1.

Zhang, S, Chen, W, Liang, C, To Evaluate the Damage of Renal Function in CIAKI Rats by fMRI and Correlate with the Expression of AQP1.  Radiological Society of North America 2014 Scientific Assembly and Annual Meeting, - ,Chicago IL. http://archive.rsna.org/2014/14014129.html