Jae Hwan Lee MD, Presenter: Nothing to Disclose

Kyu Ri Son MD, Abstract Co-Author: Nothing to Disclose

Hyo-Cheol Kim MD, Abstract Co-Author: Nothing to Disclose

The purpose of this study was to compare the in vitro drug release characteristics of DC bead and various kinds of Lipiodol emulsion, and to compare the tumor response in animal liver tumor model. 

We prepared 4 types of Lipiodol emulsion: A) 10mg of DOX in 0.5 ml of contrast media mixed with 2 ml of Lipiodol, B) 10 mg of DOX in 1.25 ml of contrast media mixed with 1.25 ml of Lipiodol, C) 10 mg of DOX in 0.5 ml of normal saline (NS) mixed with 2 ml of Lipiodol, D) 10 mg of DOX in 1.25 ml of NS mixed with 1.25 ml of Lipiodol. DC bead of 100-300 µm in diameter were loaded with DOX (37.5 mg/ml) according to the manufacturer’s instruction. Drug release from emulsions or DC bead was evaluated in in vitro model. Three weeks after implantation of VX2 carcinomas in the liver, TACE was performed using A) 4:1 volume ratio of Lipiodol and DOX solution, B) 1:1 volume ratio of Lipiodol and DOX solution, C) DC bead.

The released amounts (%) of DOX at 24 h are as follows: 20.64 ±0.20% for DC bead, 42.65 ±1.51% for Lipiodol:DOX in NS = 4:1, 45.74 ±2.14% for Lipiodol:DOX in Pamiray = 4:1, 60.92 ±1.45% for Lipiodol:DOX in NS = 1:1, and 56.91 ±2.31% for Lipiodol:DOX in Pamiray = 1:1. AUC value of group A was significantly lower than that of group B (p < 0.05), but there is no significant difference compared to that of group C. AUC value of group B was 3.43-fold higher than that of group C (p < 0.05). Cmax value of group A exhibited significant difference compared to those values of group B and C (p < 0.05). Particularly, Cmax value of group B was 12.12-fold higher than that of group C (p < 0.05). 

stable Lipiodol emulsion can be created by excessive Lipiodol mixed with DOX dissolved in contrast media. DOX release from Lipiodol emulsion depends on volume ratio of Lipiodol and DOX solution. DC bead has more sustained DOX release than Lipiodol emulsion. 

(Dealing with making effective chemoembolic mixture in TACE) DOX -contrast media mixture with excessive lipiodol  forms  more stable emulsion, and DC bead has more sustained DOX releasing capacity than Lipiodol emulsion. These knowledge may be useful in acheving effective drug delivery to HCC in TACE.  

Lee, J, Son, K, Kim, H, Comparison of Drug Release between Conventional Chemoembolization and Drug Eluting Beads Chemoembolization.  Radiological Society of North America 2014 Scientific Assembly and Annual Meeting, - ,Chicago IL. http://archive.rsna.org/2014/14007019.html