Yanji Luo, Presenter: Nothing to Disclose

Shiting Feng MD, Abstract Co-Author: Nothing to Disclose

Ziping Li MD, PhD, Abstract Co-Author: Nothing to Disclose

To develop a nano-micelle for anti-tumor drug delivery and intracellular drug release triggered by pH.

The pH sensitive copolymer PEG-P(Asp-DIP)-P(Lys-Ca) (PEALCa) was designed in our study. In order to demonstrate the target and therapeutic potential of the vesicle, cell culture experiments of the PTX-SPIO-loaded vesicles against human colorectal cancer LoVo cells were conducted. Besides, as a MRI-visible drug delivery system, the intracellular drug release of the vesicles in vivo was studied by MRI to validate the efficiency. Our study attempts to explore the transferring efficiency of PTX-PEALCa into tumoral cells and anti-cancer improvement of PTX-PEALCa compared to conventional anti-cancer drugs.

Drug release study revealed that PTX in the vesicles was released faster at pH 5.0 than at pH 7.4. Cell culture studies showed that the PTX-SPIO-PEALCa was effectively internalized by human colon carcinoma cell line (LoVo cells), and PTX could be embedded inside lysosomal compartments. Moreover, the colorectal cancer delivery effect was verified in vivo with magnetic resonance imaging (MRI) and histology analysis. Consequently effective suppression of cancer cell growth was detected. 

These results indicated that the PTX-SPION-loaded pH-sensitive vesicles were a promising MRI-visible drug release system for colorectal cancer therapy.

The pH-triggered drug release was found to efficiently amplify the intracellular drug concentration which determined the anticancer outcome. The drug-delivery effect in vivo was visible by MRI technology. The results of this study revealed the great potential of PTX-SPIO-PEALCa vesicles as a multifunctional nanomedicine for tumor therapy. 

Luo, Y, Feng, S, Li, Z, pH-Sensitive Nanomicelles for Controlled and Efficient Drug Delivery to Colorectal Cancer Cells.  Radiological Society of North America 2014 Scientific Assembly and Annual Meeting, - ,Chicago IL. http://archive.rsna.org/2014/14003972.html