Abstract Archives of the RSNA, 2013
Zien Zhou MD, Presenter: Nothing to Disclose
Weiwei Ma, Abstract Co-Author: Nothing to Disclose
Lei Li, Abstract Co-Author: Nothing to Disclose
Jia Hua, Abstract Co-Author: Nothing to Disclose
Jianrong Xu, Abstract Co-Author: Nothing to Disclose
Jiani Hu, Abstract Co-Author: Nothing to Disclose
Wenjin Wang, Abstract Co-Author: Nothing to Disclose
Yunqi Yan, Abstract Co-Author: Nothing to Disclose
The purpose of this study is to monitor different organ or tissue’s structural evolvement(hind limb, gizzard, heart, brain and eye) serially in chicken embryo during development using DTI.
Eight fertile Hy-Line White eggs were placed into an automatic digital incubator with temperature (37.8°C) and humidity (60%). From days 6 to 18, five normally developing eggs were scanned every 24 hours(T2WI and DTI). The dual-cooling technique(eggs were cooled at 4°C for one hour before imaging and wrapped in a piece of Techni-Ice during imaging) was used to suppress motion artifact.
MR scans were performed using the 3.0 T Philips Achieva System(Philips Medical Systems, Best, Netherlands) with a four-channel dedicated animal coil. The sequence parameters of T2WI and DTI are shown in table 1. All imaging planes were sagittal.
Different organ and tissues(hind limb, gizzard, heart, brain and eye) were recognized according to the anatomical position and signal difference in T2WIs, which were also used to guide the placement of ROIs for DTI analysis. FA was automatically calculated using the Dti-Studio v2.4 software (Johns Hopkins University, USA). Three ROIs were put in each organ or tissue, and the average FA was used as the structural anisotropy of that organ or tissue. The incubation day-dependent changes in structural anisotropy of these organs were obtained according to the average data of all five embryos in every experimental day.
The average FAs of the five eggs in hind limb, gizzard, heart, brain and eye from day 6 to 18 are shown in table 2. The gizzard in day 6 and the heart in day 6,7,8,9 is too small to be discriminated in DTI. The incubation day-dependent linear regression of structural anisotropy in these organ or tissues is shown in figure 1. The FA value shows a progressive linear increase in hind limb, gizzard, heart and brain with the increasing days of incubation, and the FA value in eye shows no change. Figure 2 is an example of ROIs’ drawing in hind limb, gizzard, heart, brain and eye according to T2WI and corresponding FA maps of DTI at incubation day 12, 14,17.
MRI has the potential to observe the structural development of different organ of tissue in chicken embryo without sacrifice for histological examination.
A widely-available (3.0 T) MRI system can be used as a powerful investigative tool to evaluate embryonic development with DTI.
Zhou, Z,
Ma, W,
Li, L,
Hua, J,
Xu, J,
Hu, J,
Wang, W,
Yan, Y,
In Ovo Serial Monitoring of Different Organ or Tissue's Structural Evolvement in Developmental Chicken Embryo Using DTI. Radiological Society of North America 2013 Scientific Assembly and Annual Meeting, December 1 - December 6, 2013 ,Chicago IL.
http://archive.rsna.org/2013/13044248.html