Darrell E. Hurt PhD, Abstract Co-Author: Nothing to Disclose

Lawrence J Faucette, Abstract Co-Author: Nothing to Disclose

Marcelino Bernardo BS, Abstract Co-Author: Nothing to Disclose

Jennifer Hufton, Abstract Co-Author: Nothing to Disclose

Danny Ragland, Presenter: Nothing to Disclose

Nilo A. Avila MD, Abstract Co-Author: Nothing to Disclose

This process significantly reduces the amount of histology lab time and materials, and pathologist’s time to identify and confirm areas of interest. Additionally, we can more accurately correlate tissue pathology with its corresponding imaging characteristics; however, the fixation process will alter the MR contrasts.

Immune system components may develop focal lesions in response to infectious disease. For example, lymph nodes may be altered by edema, necrosis, or hyperplastic changes.  Time and resource intensive serial sectioning of a node is typically performed to study the histopathology of these lesions. However, lesions may be more efficiently detected in non-invasive magnetic resonance (MR) imaging. In this work we use a 3D printer to produce a custom jig from 3D MR data. The jig aligns the tissue for optimum slicing to provide maximum information on the lesions of interest. To demonstrate the capability we use a 3D MR sequence to produce a node specific mold to precisely section the lesion of interest within the node.

Immune system components may develop focal lesionsin response toinfectious disease. For example, lymph nodes maybe altered by edema, necrosis, orhyperplastic changes. Time and resource intensive serial sectioning ofa node is typically performed to study the histopathology of these lesions. However, lesions may be more efficiently detected in non-invasive magnetic resonance (MR) imaging. In this work we use a 3D printer to produce a custom jig from 3D MR data. The jig aligns the tissue for optimum slicing to provide maximum information on the lesions of interest. To demonstrate the capability we use a 3D MR sequence to produce a node specific mold to precisely section the lesion of interest within the node.

This process significantly reduces the amount of histology lab time and materials, and pathologist’s time to identify and confirm areas of interest. Additionally, we can more accurately correlate tissue pathology with its corresponding imaging characteristics; however, the fixation process will alter the MR contrasts.

Hurt, D, Faucette, L, Bernardo, M, Hufton, J, Ragland, D, Avila, N, MR Guided Histopathology in Infectious Disease.  Radiological Society of North America 2012 Scientific Assembly and Annual Meeting, November 25 - November 30, 2012 ,Chicago IL. http://archive.rsna.org/2012/12028606.html