Xin Wang MD,PhD, Presenter: Nothing to Disclose

Tokomo Kato, Abstract Co-Author: Nothing to Disclose

Toshiro Inubushi PhD, Abstract Co-Author: Nothing to Disclose

Rongtian Xu, Abstract Co-Author: Nothing to Disclose

Ke Xu MD, Abstract Co-Author: Nothing to Disclose

To dedicate on tumor metabolic flux in rat C6 glioma subcuntanous transplantation models and showed the feasibility of monitoring the real-time changes of 13C-labeled glucose and lactate derived from glycolysis by using GE-HMQC in vivo at 7T.

8 male Wistar rats were involved. GE-HMQC spectroscopic imaging was performed to detect the 1H signals coupled with 13C by the 2:2:1 crusher gradient pulse. After bolus injection of D-[1-13C]-glucose through tail vein, spectral collection was started and alternated between glucose (C1H) or lactate (C3H) at invervals of 5 minutes 41seconds. The signal intensities (SI) of glucose (C1H) and lactate (C3H) were acquired in corresponding time point and normalized by initial value. The signal change per minute in one time-course and dynamic change with tumor growth (2 rats for lactate production) were also obtained.

Lactate (C3H) signal over time was detected in all eight rats, and two time courses of glucose (C1H) spectra were obtained. The SI of lactate reached maximum about 30-50 minutes and decreased back to initial state in 2-3 hours, while glucose signal reached peak during 10-20 minutes and decreased about 30 minutes after D-[1-13C]-glucose injection. With tumor growing, the SI of lactate was increased and declining process prolonged except for two time courses in the two different rats respectively.

We successfully identified the real-time courses of glucose (C1H) and lactate (C3H) by 1H-detected 13C NMR spectroscopy in vivo, as well as the changes of signal intensities with tumor growth at 7T.

The technique used in this study has the potential in tumor diagnosis, stage, as well as preceding the prognosis and effect after therapy, as changes in tumor metabolism are earlier than tumor size.

Wang, X, Kato, T, Inubushi, T, Xu, R, Xu, K, 13C-labeled Glucose Metabolism in Rat Glioma Model Monitored in Vivo Using Gradient-enhanced Heteronuclear Multiple Quantum Coherence Spectroscopic Imaging at 7T.  Radiological Society of North America 2012 Scientific Assembly and Annual Meeting, November 25 - November 30, 2012 ,Chicago IL. http://archive.rsna.org/2012/12027236.html