Ruiping Zhang MD, PhD, Presenter: Nothing to Disclose

jun xie, Abstract Co-Author: Nothing to Disclose

Graft bone marrow mesenchymal stem cells labeled superparamagnetic iron oxide (SPIO) into the spinal cord injury models via subarachnoid space, to observe the feasibility of MRI tracking of grafted cells.

BMSCs were labeled with SPIO．Rabbit spinal Cord injury models were made and the micro tubes were inserted into subarachnoid space. Rabbits were randomly divided into 3 group．Group A received SPIO labeled cells via subarachnoid spaces; Group B received unlabeled cells; Group C was injected by PBS liquid as the control group．Grafted BMSCs were tracked by Serial 1.5T MRl in 3，7 ,14 and 21 days after transplantation and compared with tissue slices of injured spinal cord．

After BMSCs labeled with SPIO were grafted into the spinal cord injury, MRI test was performed at 7, 14, 21 days respectively. At 7 days, there appeared point-like low signal shadow in the injured spinal cord segment in T2WI; At 14 days, this low signal shadow increased; At 21 days, this shadow decreased. Cells containing blue iron particles in the area of spinal cord injury could be seen in tissue sections with Prussian blue staining

Labeled BMSCs could migrate to the injured spinal cord segment after grafting via subarachnoid space, which could be tracked with MRI in vivo.

Vivo Tracking of Bone Marrow Mesenchymal Stem Cells Labeled with Superparamagnetic Iron Oxide after Spinal Cord Injury in Rabbits by Magnetic Resonance Imaging

Zhang, R, xie, j, In Vivo Tracking of Bone Marrow Mesenchymal Stem Cells Labeled with Superparamagnetic Iron Oxide after Spinal Cord Injury in Rabbits by Magnetic Resonance Imaging.  Radiological Society of North America 2012 Scientific Assembly and Annual Meeting, November 25 - November 30, 2012 ,Chicago IL. http://archive.rsna.org/2012/12021352.html