So Yeon Kim MD, Presenter: Nothing to Disclose

Siwon Kim, Abstract Co-Author: Nothing to Disclose

Chul-Woong Woo BS, Abstract Co-Author: Nothing to Disclose

Jae Ho Byun MD, Abstract Co-Author: Research Consultant, Bayer AG

Seung Soo Lee MD, Abstract Co-Author: Nothing to Disclose

Moon-Gyu Lee MD, Abstract Co-Author: Nothing to Disclose

Jai Young Cho, Abstract Co-Author: Nothing to Disclose

Suhkmann Kim, Abstract Co-Author: Nothing to Disclose

Jin Seong Lee MD, Abstract Co-Author: Nothing to Disclose

High-Resolution Magic Angle Spinning (HRMAS) 1H MR Spectroscopy (MRS) has allows metabolite profiling of intact tissues with high resolution. The purpose of our study was to explore the potential of HRMAS 1H MRS for metabolite characterization and differentiation of human hepatocelluar carcinoma (HCC) and colorectal liver metastases (CRLM).

We prospectively included pathologically confirmed 21 malignant hepatic tumors (8 HCCs and 13 CRLMs) from 21 patients who underwent resection of hepatic tumors. Using intact tissue samples from tumors and non-involved hepatic parenchyma obtained at operation room, HRMAS 1H MRS was performed at 11.7 T. All observable metabolite signals were acquired using water-presaturated standard one-dimensional Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence. Metabolomic profiles contributed to the differentiation of HCCs and CRLMs as well as the differentiation of tumors and non-involved hepatic parenchyma, were represented by orthogonal partial least squares discriminant analysis (OPLS-DA) and loading plots. Metabolite intensity normalized by total spectral intensities in both tumors was compared using student-t tests.

The separation between tumors and non-involved hepatic parenchyma was observed OPLS-DA. In addition, metabolomic characteristics of HCCs showed marked separation from those of CRLMs by OPLS-DA. Compared to CRLMs, HCCs showed elevated levels of glucose, glycine, o-phosphocholine, sn-Glycero-3-phosphocholine, and glutamine (P < 0.05), and decreased levels of hypoxanthine (P = .01).  

HRMAS 1H MRS has the ability to metabolically characterize HCCs and CRLMs, and could be very useful to differentiate HCC from CRLM.  

Key differences in the metabolite profiles identified on HRMAS 1H MRS would help us to understand molecular pathways and apply in vivo MRS for non-invasive evaluations of hepatic tumors.

Kim, S, Kim, S, Woo, C, Byun, J, Lee, S, Lee, M, Cho, J, Kim, S, Lee, J, Metabolomic Differentiation between Hepatocelluar Carcinoma and Hepatic Metastases from Colorectal Cancer with High-Resolution Magic Angle Spinning 1H MR Spectroscopy.  Radiological Society of North America 2011 Scientific Assembly and Annual Meeting, November 26 - December 2, 2011 ,Chicago IL. http://archive.rsna.org/2011/11012898.html