Yonggang Li MD, Presenter: Nothing to Disclose

Liang Guo, Abstract Co-Author: Nothing to Disclose

To investigate in vivo and in vitro 1H MRS features of normal muscle tissues and VX2 soft tissue tumors in rabbits and to determine whether in vivo 1H MRS of soft tissue tumors is feasible.

20 Newzealand white rabbits were implanted with 0.2ml VX2 tumor tissue suspension in the right proximal thighs. We performed MRI and 1.5T 1H MRS and analyzed the 1H MRS features of normal muscle tissue and VX2 soft tissue tumors in all rabbits before implantation and 40 days after implantation respectively. Cho/Cr and Lipid/Cr were calculated on workstation and a student’s t-test of significance was done. After the animals were sacrificed, in viro 14.1T 1H MRS of the fresh sample of normal muscle and VX2 soft tissue tumors and its correlation with in vivo 1H MRS findings were performed.

Cho, Cr and lipid peak were seen in the in vivo 1H MRS of normal muscle and VX2 soft tissue tumors in all rabbits. In vivo 1H MRS in VX2 soft tissue tumors showed prominent Cho peak and decreased Lipid peak. The average values of Cho/Cr of the VX2 soft tissue tumors and the normal muscles were 1.7572±0.3478 and 0.6432±0.1763 and the average values of Lipid/Cr were 1.2209±0.3412 and 7.6723±2.7864 respectively. The differences of Cho/Cr and Lipid/Cr between VX2 soft tissue tumors and normal muscles were statistically significant(P<0.01). In contrast to normal muscle, in vitro 1H MRS revealed increased Ala, Glu, Cho, PC, Lac, mI and Gly peak and decreased Lipid and Glc peak in all cases of the VX2 soft tissue tumor. In vivo 1H MRS changes of Cho and Lipid in th VX2 soft tissue tumors was consistent with in vitro 1H MRS.

The normal muscle and VX2 soft tissue tumor of rabbits showed great difference in 1H MRS. In vivo 1H MRS findings demonstrated great consistence with that of in vitro 1H MRS. In vivo 1H MRS can reveal the metabolism information of the soft tissue tumors and it is feasible to diagnose the soft tissue tumors.

In vivo 1H MRS can reveal the metabolism information of the soft tissue tumors and it is helpful in the differentiation of malignant and benign soft tissue tumors.

Li, Y, Guo, L, In Vivo 1.5T and ex Vivo 14.1T 1H MR Spectroscopy Study of VX2 Soft Tissue Tumors in Rabbits.  Radiological Society of North America 2007 Scientific Assembly and Annual Meeting, November 25 - November 30, 2007 ,Chicago IL. http://archive.rsna.org/2007/5015761.html