Tianlong Huo, Presenter: Nothing to Disclose

Xiangke Du, Abstract Co-Author: Nothing to Disclose

This study was to develop a novel MR probe containing arginine-glycine-aspartic acid (RGD) peptide ligand for imaging avb3 integrin receptor-expressed tumor .

Commercially available HYNIC-RGD conjugate with co-ligand EDDA was labeled by Gd3+ in a form of GdCl3 at room temperature for 2 hours and the mixture was isolated and purified by SPE/HPLC to get entire probe Gd-HYNIC/EDDA-RGD. Human HCC cell line BEL-7402 was cultured in DMEM containing 10% FBS in a 37o incubator with 5% CO2.The cells harvested and suspended in serum-free DMEM at a concentration of 1×107cells/ml were transplanted into the right axillary space of the 4-week age male nude mice. Gd-HYNIC/EDDA-RGD cell binding assay to avb3 integrin receptor and its toxicity to the living cells experiment were performed. The in vivo imaging of the four arms of xenografts (6 mice per arm，injected respectively Gd-HYNIC/EDDA-RGD，Gd-HYNIC/EDDA，Gd-DTPA solutions and saline with a identical dosage of 0.1mmol.kg-1 in a same injection volume of 0.2 ml) were performed at a 1.5T MR unit with a dedicated animal coil following the same protocol scanning at 0, 30,60,90,120,180,240 minutes post-intravenous injection (p.i.). And four arms of nude mice then were sacrificed for pathological examinations to testify the imaging results.

Gd-HYNIC/EDDA-RGD was successfully isolated and purified by SPE/HPLC and was verified validity on signal enhancement in vitro and in vivo experiments. Drug toxicity trial showed there is no significant adverse effect on living cells regards to proliferation and the other routine metabolic activities. The nude mice model bearing HHCC was well established. There was over 30% signal enhancement on T1WI FSE images at 90 minute post-intravenous injection of the Gd-HYNIC/EDDA-RGD compared with the baseline, and the signal to time curve is straightforward over time in the span of 0 to 90 minute p.i., while the control arms do not show this tendency.

Gd-HYNIC/EDDA-RGD has potential to serve as a MR probe detecting avb3 integrin receptor-expressed tumors.

This work may add new possibility to early detecting and differentiating specific tumors.

Huo, T, Du, X, Gd-HYNIC/EDDA-RGD as an MR Probe for Imaging Human Hepatocellular Carcinoma Xenografts in Nude Mice Model.  Radiological Society of North America 2007 Scientific Assembly and Annual Meeting, November 25 - November 30, 2007 ,Chicago IL. http://archive.rsna.org/2007/5014457.html