Discovery of the Imaging Markers of Human Melanoma Metastatic Potential in Mouse Xenografts by NADH/Fp Fluorescence Imaging

SSC05-05

Discovery of the Imaging Markers of Human Melanoma Metastatic Potential in Mouse Xenografts by NADH/Fp Fluorescence Imaging

Scientific Papers

Presented on November 27, 2006
Presented as part of SSC05: Molecular Imaging (Applications I)

Molecular Imaging Travel Award

Lin ZJ Li PhD, Presenter: Nothing to Disclose

Lily Moon, Abstract Co-Author: Nothing to Disclose

Tuoxiu Zhong, Abstract Co-Author: Nothing to Disclose

Rong Zhou PhD, Abstract Co-Author: Nothing to Disclose

Mary Hendrix, Abstract Co-Author: Nothing to Disclose

Dennis Leeper, Abstract Co-Author: Nothing to Disclose

Chance Britton, Abstract Co-Author: Nothing to Disclose

Jerry D. Glickson, Abstract Co-Author: Nothing to Disclose

et al, Abstract Co-Author: Nothing to Disclose

The long term objective of this study is to evaluate optical imaging methods that could be applied clinically to distinguish between aggressive and indolent forms of melanoma in human. Low temperature NADH/Fp fluorescence imaging has been used for the measurement of redox ratio in mitochondria respiration. In this study, we would like to apply this method to image mouse xenografts of human melanoma, and see if we can find imaging markers of human melanoma metastatic potential.

We have established xenografts in athymic nude mice of the human melanoma cell lines of various levels of metastatic potential, C8161, A375P, A375M, A375P5, A375P10. Their invasive abilities have been measured in vitro using a basal membrane in the rank order of A375P

RESULTS

We observed that the more aggressive xenografts had significantly higher Fp redox ratios [Fp/(Fp+NADH)] than the indolent ones. A bi-modal spatial distribution of redox ratios was observed in both C8161 and A375M tumors. The central region of these tumors is significantly more oxidized than the outer rim. The average Fp redox ratios of the tumor cores are 0.9, 0.65, 0.3 for C8161, A375M and A375P, respectively, following the rank order of invasive abilities C8161 (12.5%)>A375M(5%)>A375P(3%). The mean value of H&E histological staining of C8161 tumors also showed that the tumor core had more apoptotic cells than the rim.

CONCLUSION

These findings indicate that the Fp redox ratio can be a potential marker of human melanoma aggressiveness or metastatic potential.

CLINICAL RELEVANCE/APPLICATION

The method can be used to examine clinical biopsy samples. If the results of this study can be generalized to other cancer lines, the method will have high clinical sginificance.

Cite This Abstract

Li, L, Moon, L, Zhong, T, Zhou, R, Hendrix, M, Leeper, D, Britton, C, Glickson, J, et al, , Discovery of the Imaging Markers of Human Melanoma Metastatic Potential in Mouse Xenografts by NADH/Fp Fluorescence Imaging. Radiological Society of North America 2006 Scientific Assembly and Annual Meeting, November 26 - December 1, 2006 ,Chicago IL. http://archive.rsna.org/2006/4441561.html