Ming Qiang Huang PhD, Abstract Co-Author: Nothing to Disclose

Seung-cheol Lee, Abstract Co-Author: Nothing to Disclose

David S. Nelson, Abstract Co-Author: Nothing to Disclose

Harish Poptani PhD, Abstract Co-Author:

Stephen Pickup, Abstract Co-Author: Nothing to Disclose

Jerry D. Glickson, Presenter: Nothing to Disclose

E. J. Delikatny, Abstract Co-Author: Nothing to Disclose

et al, Abstract Co-Author: Nothing to Disclose

Evaluate the availability of 1H and 31P MRS for response detection of the most common subtype of Non-Hodgkin's Lymphoma (NHL), diffuse large B-cell lymphoma (DLCL2), xenografts in SCID mice to combination chemotherapy with multiple cycles of CHOP (cyclphosphamide, hydroxydoxorubicin, oncovin, prednisone).

DLCL2 tumors were subcutaneously implanted in the upper thighs of 6-8 week old female SCID mice by inoculating 10.0×106 DLCL2 cells. The tumor-bearing mice were treated with 4 cycles of CHOP plus bryostatin 1. Since DLCL2 over-expresses the mdr1 gene, which may affect response to some of the components of CHOP, Bryostatin 1 was used to inhibit expression of this gene for better therapeutic response. NMS were performed with a 9.4 T (89 mm diameter) vertical bore magnet interfaced to a Varian Inova console prior to and after the first treatment. A slotted-tube 1H and 31P double-tuned probe (inner diamete, 10 mm) was used for transmission and detection. A non-localized selective multiple-quantum coherence transfer sequence (Sel-MQC) was employed to detect the global lactate and total choline (TCho) signals from the tumor. Non-localized 31P MRS was performed to evaluate tumor energy and phospholipid metabolism.

The tumor volumes in the CHOPB treated group decreased to about 60% of the pre-treatment values, while the volumes of the control tumors increased monotonically. The lactate and TCho levels in 1H MR spectra as well as the PME/βNTP ratios in 31P MR spectra of the tumors in the CHOP + Bryostatin (CHOPB) treated animals began to decrease approximately one week after the first cycle of treatment; while, these levels of the untreated control tumors increased slightly. In comparison with CHOP, CHOPB produced larger and earlier spectral changes. A decrease in TCho was observed after CHOPB but not after CHOP.

1H and 31P MRS has been performed to non-invasively assess the response of DLCL2 xenografts in SCID mice to CHOP chemotherapy.

Our results suggest that the combination of 1H and 31P MRS could be a sensitive modality for in vivo detection of therapeutic response of NHL tumors.

Huang, M, Lee, S, Nelson, D, Poptani, H, Pickup, S, Glickson, J, Delikatny, E, et al, , In Vivo Detection of Therapeutic Response of NHL Xenograft to Chemotherapy by ¹H & ³¹P MRS.  Radiological Society of North America 2006 Scientific Assembly and Annual Meeting, November 26 - December 1, 2006 ,Chicago IL. http://archive.rsna.org/2006/4437993.html