Aude Brule, Presenter: Nothing to Disclose

Nicolas Elie PhD, Abstract Co-Author: Nothing to Disclose

Michele Lamuraglia MD, Abstract Co-Author: Nothing to Disclose

Nathalie Dehez, Abstract Co-Author: Nothing to Disclose

Nathalie Brigitte Lassau MD, PhD, Abstract Co-Author: Nothing to Disclose

Pierre Peronneau PhD, Abstract Co-Author: Nothing to Disclose

3D reconstruction of tumor morphological and vascular features with high-frequency ultrasonography and quantification over time of the perfused tissue volume from raw data using perfusion software.

Five mice received subcutaneous injections of murine melanoma cells (B16). Ultrasound and Doppler examinations took place on D+7,9,11,14,16,18 with an Aplio® ultrasonograph (Toshiba) equipped with 9 and 14MHz probes. A series of 2D parallel slices, spaced 0.5mm apart, were acquired by mechanical scanning in B and Doppler modes. Taking into account the thickness of the ultrasonic beam and tumor dimensions, perfusion was detected only on 3 transverse sections of each tumor, regularly spaced from head to tail, in order to obtain the average tumor perfusion volume. Contrast agent (SonoVue®,Bracco) was injected three times per tumor and perfusion was measured in “Vascular Recognition Imaging” (VRI, Toshiba) mode. Contrast uptake was quantified by processing raw data using the Chi-Q (Toshiba) perfusion software. Perfusion data were analyzed by modeling the contrast uptake curve using a sigmoid equation. Two indices were computed from these curves : peak intensity and time to peak intensity for each section. The average of the 3 sections of each parameter was computed and normalized according to the considered surface. Tumors were reconstructed in 3D (vascularization, perfusion) with AMIRA-3D (TGS) software.

Peak intensity and time to peak intensity values differed significantly according to the position of the three sections (up to 1000%) emphasizing the need to take into account the entire volume of tumor perfusion. The time to peak intensity of the mean volumic tumor perfusion increased the first days (16s) and then decreased (10s) while peak intensity decreased during time of observation (around 0.005 to 0.0001).

This work shows the interest of studying contrast uptake in 3D from raw data in order to accurately quantify tissue perfusion during tumor growth.

Brule, A, Elie, N, Lamuraglia, M, Dehez, N, Lassau, N, Peronneau, P, 3D Quantification of Tumor Contrast Agent Perfusion over Time with Ultrasonography.  Radiological Society of North America 2005 Scientific Assembly and Annual Meeting, November 27 - December 2, 2005 ,Chicago IL. http://archive.rsna.org/2005/4414023.html