Adel Maataoui MD, Abstract Co-Author: Nothing to Disclose

Jun Qian MD, Abstract Co-Author: Nothing to Disclose

Mohammad Fawad Khan MD, Abstract Co-Author: Nothing to Disclose

Daryusch Vossoughi, Abstract Co-Author: Nothing to Disclose

Thomas Josef Vogl MD, Presenter: Nothing to Disclose

The present study is to access whether the effectiveness of a combination of transarterial chemoembolization (TACE) and arterial administration of TNP-470, an inhibitor of angiogenesis in the liver is superior to TACE alone in the treatment of ACI rats with hepatocellular carcinoma.

Tumor cells of Morris Hepatoma 3924A, hepatocellular carcinoma (5×106) were implanted subcutaneously at the flank of a male ACI rat. 13 days later, the subcutaneous growing solid tumor was explanted from the donor animal and cut into small cubes of about 2 mm3 volume. Afterwards, the tumor fragments were inserted into the subcapsular pocket of the liver in 20 male ACI rats. 14 days after implantation, these 20 rats were randomised into one of two groups: Group A (n=10) include rats who had TACE (Mitomycin 0.1 mg  Lipiodol 0.1 ml) with arterial administration of TNP-470 in the liver (5.0 mg) and Group B (n=10) include rats who had only TACE (Mitomycin 0.1 mg  Lipiodol 0.1 ml). Before and 13 days after, the tumor volume (V1 and V2) were determined by MRI and the tumor growth ratio (V2/V1) was calculated.

The mean tumor volume before and after therapy (V1 and V2) were 0.0370 cm3 and 0.1476 cm3 in group A, 0.0380 cm3 and 0.3398 cm3 in group B, respectively. The mean V2/V1 were 4.11 in group A and 9.14 in group B, respectively. Compared to group B (TACE alone), group A (combination of TACE and arterial administration of TNP-470) showed a significant inhibition of tumor growth (P<0.01). None of the animals died during implantation or in the postoperative period. Necrosis, hemorrhage and metastases of the tumor were not observed in all groups.

These results suggest that TACE combined with arterial administration of TNP-470 in the liver may enhance the antitumor effect of TACE alone in the treatment of HCC in rats.

Maataoui, A, Qian, J, Khan, M, Vossoughi, D, Vogl, T, The Antitumor Effect of TNP-470 in Transarterial Chemoembolization in an Animal Model of Hepatocellular Carcinoma.  Radiological Society of North America 2004 Scientific Assembly and Annual Meeting, November 28 - December 3, 2004 ,Chicago IL. http://archive.rsna.org/2004/4416531.html