Wu Hanping MD, Presenter: Nothing to Disclose

Feng Gansheng, Abstract Co-Author: Nothing to Disclose

Liang Huimin, Abstract Co-Author: Nothing to Disclose

This study was to explore the feasibility of VEGF antisense oligodeoxynucleotides (ASODN) inhibiting angiogenesis after TAE on rat liver cancer model.

The cellular distribution of 5'-FITC labeled VEGF ASODN was observed by fluorescence microscopy. ASODNs were cultured with Walker-256 cells, the VEGF concentrations were detected by ELISA 48h later. 5'-FITC labeled ASODN mixed with (mixed group, n=6) or without (TAI group, n=6) lipiodol (LP) was administrated via hepatic artery in Walker-256 transplanted rat liver tumor model. The distributions of ASODN in liver, lung and kidney tissue were observed by fluorescent microscopy. Other 30 liver tumor rats were divided into 3 groups. 0.2ml LP (LP group, n=10), 3OD ASODN mixed with 0.2ml LP (LP+ODN group, n=10) and 0.2ml normal saline (control group, n=10) were infused into the hepatic artery. The volumes of tumors were measured before and 7 days after treatment. VEGF mRNA was detected by RT-PCR. The microvessel density (MVD) and VEGF expression were observed by immunohistochemistry.

ASODN could enter the Walker-256 cell cytoplasm within 2h and enter the nuclei within 4h, disappear at 48h. ASODN inhibited Walker-256 cells' VEGF expression. The fluorescence stained stronger and stayed longer in liver in the mixed group than TAI group. The tumor growth rate was significantly lower in LP+ODN group than that of LP and control groups (140.1%±33.8%, 177.9%±64.9% and 403.9%±69.4% respectively, F=60.02, P0.05). The MVD in LP+ODN group (53.1±18.4) was significantly less than that of control group (73.2±20.4) and LP group (80.3±18.5) (F=5.44, P<0.05)

VEGF ASODN could transfect and inhibit VEGF expression of Walker-256 cells. ASODN Mixed with lipiodol via hepatic artery is an ideal way treating liver carcinoma, and decrease liver cancer growth, VEGF expression and microvessel density more than LP alone.

Hanping, W, Gansheng, F, Huimin, L, The Experimental Study of VEGF Antisense Oligodeoxynucleotides with Lipiodol in Arterial Embolization of Liver Cancer in Rats.  Radiological Society of North America 2004 Scientific Assembly and Annual Meeting, November 28 - December 3, 2004 ,Chicago IL. http://archive.rsna.org/2004/4402959.html