RSNA 2005 

Abstract Archives of the RSNA, 2005


LPL10-06

Labelling Efficiency of Iron Oxide Nanoparticles in Mesenchymal Stem Cell: Comparison with MR Signal Intensity

Scientific Posters

Presented on November 30, 2005
Presented as part of LPL10: Physics (Cellular Imaging, MR Imaging)

Participants

Kyu-Ri Son MD, Presenter: Nothing to Disclose
Woo-Kyoung Moon, Abstract Co-Author: Nothing to Disclose

PURPOSE

To compare labelling efficiency of nanoparticles and MR signal intensity in human mesenchymal stem cells.

METHOD AND MATERIALS

In the present study, we compared three different SPION such as standard superparamagnetic iron oxides (feridex), monocrystalline iron oxide nanoparticles (MION-47), and crosslinked iron oxides with tat peptide (tat-CLIO) in terms of their capacity to label human mesenchymal stem cells(hMSC). hMSC were incubated in cell culture media containing various SPION with which concentrations and incubation times have been modified in previous studies. Cell labeling with iron was assessed by Prussian blue stain and iron contents of the cell were analyzed by atomic absorption furnace spectrophotometer (AAS). And intracellular cytoplasmatic accumulation in vesicles was evaluated using electron microscopy. Proliferation and vitality of hMSCs after SPION labeling were also evaluated. The localization of iron oxides in MSC was investigated by labeling of various SPION.Furthermore, the retention of SPION or long term survival in the cells were evaluated at different incubation time points including 6, 12,24hr,7day, 15day. And human mesenchymal cells(hMSC), labeled with three SPIONs underwent MR imaging analyzed by measuring MR signal intensity and R1 and R2* relaxation rates of labeled cells and nonlabelled cells.

RESULTS

Three nanoparticles have different labelling efficiency without transfection agent,and cellular uptake of SPIONs was highest for CLIO-tat,followed by Feridex, MION-47,in decreasing order. But there is no differences of labelling efficiency between three SPONs with transfection agent. Incorporation of the three SPION appeared not to affect cell proliferation and vitality.

CONCLUSION

Clio-tat or Feridex is superior to MION in labelling efficiency to hMSC without transfection agent. And nanoparticles has little effect on cell viabilty or proliferation. Human mesenchymal stem cells(hMSCs) can be labeled with MR contrast angents and can be dipicted with a standard 1.5T MR imager.

PURPOSE

To compare labelling efficiency of nanoparticles and MR signal intensity in human mesenchymal stem cells.

METHOD AND MATERIALS

In the present study, we compared three different SPION such as standard superparamagnetic iron oxides (feridex), monocrystalline iron oxide nanoparticles (MION-47), and crosslinked iron oxides with tat peptide (tat-CLIO) in terms of their capacity to label human mesenchymal stem cells(hMSC). hMSC were incubated in cell culture media containing various SPION with which concentrations and incubation times have been modified in previous studies. Cell labeling with iron was assessed by Prussian blue stain and iron contents of the cell were analyzed by atomic absorption furnace spectrophotometer (AAS). And intracellular cytoplasmatic accumulation in vesicles was evaluated using electron microscopy. Proliferation and vitality of hMSCs after SPION labeling were also evaluated. The localization of iron oxides in MSC was investigated by labeling of various SPION.Furthermore, the retention of SPION or long term survival in the cells were evaluated at different incubation time points including 6, 12,24hr,7day, 15day. And human mesenchymal cells(hMSC), labeled with three SPIONs underwent MR imaging analyzed by measuring MR signal intensity and R1 and R2* relaxation rates of labeled cells and nonlabelled cells.

RESULTS

Three nanoparticles have different labelling efficiency without transfection agent,and cellular uptake of SPIONs was highest for CLIO-tat,followed by Feridex, MION-47,in decreasing order. But there is no differences of labelling efficiency between three SPONs with transfection agent. Incorporation of the three SPION appeared not to affect cell proliferation and vitality.

CONCLUSION

Clio-tat or Feridex is superior to MION in labelling efficiency to hMSC without transfection agent. And nanoparticles has little effect on cell viabilty or proliferation. Human mesenchymal stem cells(hMSCs) can be labeled with MR contrast angents and can be dipicted with a standard 1.5T MR imager.

Cite This Abstract

Son, K, Moon, W, Labelling Efficiency of Iron Oxide Nanoparticles in Mesenchymal Stem Cell: Comparison with MR Signal Intensity.  Radiological Society of North America 2005 Scientific Assembly and Annual Meeting, November 27 - December 2, 2005 ,Chicago IL. http://archive.rsna.org/2005/4417058.html